ThiU

• Description: thiamine ABC transporter (binding protein)

Gene name thiU Synonyms ykoF Essential no Product thiamine ABC transporter (binding protein) Function thiamine uptake Gene expression levels in SubtiExpress: thiU Interactions involving this protein in SubtInteract: ThiU Metabolic function and regulation of this protein in SubtiPathways: thiU MW, pI 21 kDa, 6.046 Gene length, protein length 600 bp, 200 aa Immediate neighbours thiV, ykoG Sequences Protein DNA DNA_with_flanks Genetic context Expression at a glance PubMed

ABC transporters, biosynthesis of cofactors, membrane proteins

Thi-box

Basic information

• Locus tag: BSU13240

Phenotypes of a mutant

Database entries

• BsubCyc: BSU13240

• DBTBS entry: no entry

• SubtiList entry: [1]

Additional information

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • ThiW-(ThiV-ThiX)-ThiU PubMed

• Localization: membrane associated (via ThiV-ThiX) PubMed

Database entries

• BsubCyc: BSU13240

• Structure: 1SBR (complex with thiamine), 1S99

• UniProt: O34911

• KEGG entry: [2]

• E.C. number:

Additional information

• Operon: thiU-thiV-thiW-thiX PubMed

• Expression browser: thiU PubMed

• Sigma factor:

• Regulation:
  • repressed by thiamine (Thi-box) PubMed

• Regulatory mechanism:
  • Thi-box: RNA switch PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 257 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 745 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1463 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 433 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 540 PubMed

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Ghislain Schyns, Sébastien Potot, Yi Geng, Teresa M Barbosa, Adriano Henriques, John B Perkins Isolation and characterization of new thiamine-deregulated mutants of Bacillus subtilis. J Bacteriol: 2005, 187(23);8127-36 [PubMed:16291685] [WorldCat.org] [DOI] (P p)

Yancho Devedjiev, Yogesh Surendranath, Urszula Derewenda, Alexandra Gabrys, David R Cooper, Rong-guang Zhang, Lour Lezondra, Andrzej Joachimiak, Zygmunt S Derewenda The structure and ligand binding properties of the B. subtilis YkoF gene product, a member of a novel family of thiamin/HMP-binding proteins. J Mol Biol: 2004, 343(2);395-406 [PubMed:15451668] [WorldCat.org] [DOI] (P p)

Dmitry A Rodionov, Alexey G Vitreschak, Andrey A Mironov, Mikhail S Gelfand Comparative genomics of thiamin biosynthesis in procaryotes. New genes and regulatory mechanisms. J Biol Chem: 2002, 277(50);48949-59 [PubMed:12376536] [WorldCat.org] [DOI] (P p)